Domaine Berthaut has been a family winery for at least 7 generations. The story begins at the end of the 18th century with François Berthaut's father and a few hectares of vines in Fixin, at 9 Rue Noisot. Then came Firmin followed by Bernard, who died early. His wife Jeanne ran the estate until their son Guy came of age. Guy took over the estate in 1930. In the aftermath of the war, Guy began to market his wines in bottles, enlarging the property with the purchase of the house at 18 rue Noisot and the rental of new sharecropping plots in the town of Gevrey-Chambertin.
Vincent and Denis Berthaut took over from their father Guy in 1974. During their careers, Vincent and Denis extended the estate by purchasing vines in Fixin. Amélie, daughter of Denis, took over the family business in 2013.